Journal of Microbial & Biochemical Technology

Mehta Praveen Kumar

Publications

• Research Article
  Production and Characterization of Acyl Transfer Activity of Amidase from Alcaligenes sp. MTCC 10674 for Synthesis of Hydroxamic Acids
  Author(s): Bhatia Ravi Kant, Bhatia Shashi Kant, Mehta Praveen Kumar and Bhalla Tek ChandBhatia Ravi Kant, Bhatia Shashi Kant, Mehta Praveen Kumar and Bhalla Tek Chand
  
  Alcaligenes sp. MTCC 10674 has been isolated as a nitrile degrading bacterium from the soil. Amidase of this organism exhibited dual activity i.e. hydrolase as well as acyl transfer. The acyl transfer activity of this organism has been used for the synthesis of variety of hydroxamic acids. Optimization of physiochemical parameters resulted into 30 folds increase in the acyl transfer activity of amidase (0.039 Umgdcm-1 to 1.17 Umgdcm-1). The acyl transfer activity of amidase of this organism has broader substrate affinity ranging from a variety of aliphatic amides (formamide, acetamide, propanamide etc.) to aromatic amides (benzamide, mandelamide, nicotinamide etc.) along with hydroxylamine for the biotransformation of these amides into corresponding hydroxamic acid. This enzyme is quite stable at 50°C with t1/2 for 8h and at 60°C this amidase have t1/2 for 1.30 h. The acyl tra.. View More»
  DOI: 10.4172/1948-5948.1000090

  Abstract PDF