Masoud Hamidi
West Indies
Research Article
Optimization of Total Carotenoid Production by Halorubrum Sp. TBZ126
Using Response Surface Methodology
Author(s): Masoud Hamidi, Malik Zainul Abdin, Hossein Nazemyieh, Mohammad Amin Hejazi and Mohammad Saeid HejaziMasoud Hamidi, Malik Zainul Abdin, Hossein Nazemyieh, Mohammad Amin Hejazi and Mohammad Saeid Hejazi
Carotenoids are one of the most diverse and broadly distributed classes of pigments in nature with a high number of biotechnological applications. Carotenoids have a broad range of functions, especially in relation to human health and their role as biological antioxidants. The increasing demand for consumption of natural carotenoids has raised interest in their bio-production. The objective of the present study was the analysis of environmental factors (temperature, pH and salinity) through response surface methodology (RSM) on the total carotenoid production of Halorubrum sp. TBZ126. In addition the effect of light was evaluated. Five levels of temperature, pH, and salinity were selected based on central composite design (CCD) and RSM to reach the optimum values for the cell growth and carotenoid production. Bio-production was carried out in an orbital shaker using a 10% (v/v) inocul.. View More»
DOI:
10.4172/1948-5948.1000158