Journal of Biomolecular Research & Therapeutics

Manzoor Ahmad Gatoo

Publications

• Research Article
  In Vitro DNA-Binding, Cleavage Activity with pBR322, Molecular Docking and Antiproliferative Studies of Newly Synthesized Steroidal Imidazolidine Hybrids
  Author(s): Ayaz Mahmood Dar, Shamsuzzaman, Mir Shabeer Ahmad and Manzoor Ahmad GatooAyaz Mahmood Dar, Shamsuzzaman, Mir Shabeer Ahmad and Manzoor Ahmad Gatoo
  
  New steroidal imidazolidine derivatives (7-9) were synthesized by reacting steroidal thiosemicarbazones (4-6) with ethyl-2-chloroacetate in absolute ethanol. After characterization by spectral and analytical data, the interaction studies of compounds (7-9) with DNA were carried out by UV-vis, luminescence spectroscopy, circular dichroism and gel electrophoresis. The compounds bind to DNA preferentially through electrostatic and hydrophobic interactions with Kb; 2.07 � 104 M-1, 2.1 � 104 M?1 and 1.9 � 104 M?1, respectively indicating the higher binding affinity of compound 8 towards DNA. Gel electrophoresis demonstrated that the compound 8 show strong interaction with DNA and during its cleavage activity with pBR322 DNA, it seems to follow the mechanistic pathway, involving singlet oxygen and superoxide anion to generate ROS responsible for initiating DNA strand scission. The docking stud.. View More»
  DOI: 10.4172/2167-7956.1000131

  Abstract PDF