Journal of Microbial & Biochemical Technology

Manohar Shinde

Publications

• Research Article
  Orthogonal Array Approach for Optimization of Carrageenase Production by Solid State Fermentation of Pseudomonas aeruginosa ZSL-2
  Author(s): Ziayoddin M, Manohar Shinde and Junna LalithaZiayoddin M, Manohar Shinde and Junna Lalitha
  
  The optimization of solid-substrate medium and fermentation conditions for the production of enzyme carrageenase by a Pseudomonas aeruginosa ZSL-2 was achieved for the first time by employing an orthogonal array testing strategy (OATS). Of the four different substrates tested, wheat bran showed maximum production of carrageenase enzyme. The factors that influence the solid-substrate fermentation (SSF) conditions such as, moisture content, moistening agent, temperature, pH, inoculum size, additional carbon source and the fermentation period for the production of enzyme were studied by one-factor at a time and also by L9(34) an orthogonal array method. The maximal production of k-carragenase (7.44 U/g) dry bacterial bran was achieved at moisture level 1:2.5 (w/v; wheat bran to mois.. View More»
  DOI: 10.4172/1948-5948.1000078

  Abstract PDF