M Sivarajan

Publications
  • Research Article
    Penicillin Production from Transformed Protoplast of Penicillium chrysogenum by Fermentation

    Author(s): M Sukumar, M Sundar and M SivarajanM Sukumar, M Sundar and M Sivarajan

    The Protoplast was prepared from the Penicillium chrysogenum and confirmed by microscopic observation and strains from the Penicillium chrysogenum were intrafused and protoplast was regenerated. The Penicillin was produced by fermentation method. The DNA was isolated from the Sclerotium rolfsii and it was characterized by electrophoresis and Protoplast of Penicillium chrysogenum was transformed with DNA of Sclerotium rolfsii. Penicillin was produced from transformed protoplast of Penicillium chrysogenum and at last HPLC was used to quantify the penicillin. Penicil - lin was isolated and purified and injected into experimental animals, where it was found not only to cure infections but also to possess incredibly low toxicity for the animals. This fact ushered into being the age of antibiotic chemotherapy, and an intense search for similar antimicrobial .. View More»
    DOI: 10.4172/2153-0645.1000102

    Abstract