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M P Shah
M P Shah
Industrial Waste Water Research Laboratory, Division of Applied and Environmental Microbiology, Enviro Technology Limited, Ankleshwar-393002 Gujarat,
India
Publications
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Research Article
Community Analysis of Ammonia Oxidizing Bacteria through Molecular Genetics in Activated Sludge of Effluent Treatment Plant
Author(s): M P ShahM P Shah
We investigated the communities of Ammonia-Oxidizing Bacteria (AOB) in activated sludge using Polymerase Chain Reaction (PCR) followed by Terminal Restriction Fragment Length Polymorphism (T-RFLP), cloning, and sequencing of the alpha-subunit of the ammonia monooxygenase gene (amoA). In this study the techniques of specific amplification of ammonia oxidiser 16S rDNA fragments by PCR, separation of mixed PCR samples by Denaturing Gradient Gel Electrophoresis (DGGE), and band identification by specific hybridization with oligonucleotide probes were combined to allow for the comparison of the community composition of multiple samples over space and time. DGGE bands of interest were also excised for DNA isolation, reamplification, sequence determination and phylogenetic analysis. We compared monthly samples by the emergent macrophyte Glyceria maxima to determine the seasonal effects that .. View More»
DOI: 10.4172/1948-5948.S5-002