Lopes F
Development of Biotechnological Processes Laboratory, Biotechnological Processes Department, University of Campinas, P.O. Box 6066, 13081-970, Campinas, SP,
Brazil
Research Article
Thermo-Stable Xylanases from Non Conventional Yeasts
Author(s): Lopes F, Motta F, Andrade C C P, Rodrigues M I and Maugeri-Filho FLopes F, Motta F, Andrade C C P, Rodrigues M I and Maugeri-Filho F
Xylanase is a commercial enzyme that has considerable applications in different types of industries, such as the feed, food, textile and paper industries, amongst others. The main goal of the present work was to select and evaluate the enzyme production from isolated wild yeasts strains obtained from several different Brazilian regions. From a total of 349 strains, two were selected, namely LEB-AAD 5 and LEB-AY 10 , which produced fairly stable enzymes. The characterization studies showed that the strain LEB-AAD 5 produced an enzyme with the following optimal range: temperature from 57.5 to 67.5 ºC and pH from 4.7 to 5.5, with a half-life of 21.33 hours at 52ºC and pH 5.3, Vmax of 1.77 μmol/mL.min and Km of 0.44 g/L. The enzyme from strain LEB-AY 10 showed the following optimal range: pH from 4.1 to .. View More»
DOI:
10.4172/1948-5948.1000049