Journal of Bioequivalence & Bioavailability

Lizhi Liu

Publications

• Research Article
  An In vitro Cell-Based LDL Uptake Model for Screening PCSK9 Modulators
  Author(s): Weiming Xu and Lizhi LiuWeiming Xu and Lizhi Liu
  
  PCSK9 plays a critical role in cholesterol metabolism by controlling the level of low-density lipoprotein receptor(LDLR). Recent population genetic studies have shown that PCSK9 is a genetic validated target for the reduction of LDLc. Several anti-PCSK9 antibodies are currently undergoing Phase II/III trails in patients with hypercholesterolemia with promising results. However, developing novel compounds that inhibit PCSK9 function is pharmaceutically preferred over both antibody and siRNA routes. We have recently developed a cell-based, functional assay incorporating recombinant PCSK9 protein for high-throughput screening of human liver cell HepG2. A pilot screen of the NINDS compound library has been successful in identifyng several potential compounds in duplicate screening. One of the lead compounds, colchicine, has been further validated with the Western blot assay for PCSK9-medi.. View More»
  DOI: 10.4172/jbb.10000168

  Abstract PDF