Journal of Stem Cell Research & Therapy

Lei Wang

Publications

• Research Article
  Induced Pluripotent Stem Cells derived Muscle Progenitors Effectively Mitigate Muscular Dystrophy through Restoring the Dystrophin Distribution
  Author(s): Wen-Feng Cai, Wei Huang, Lei Wang, Jia-Peng Wang, Lu Zhang, Muhammad Ashraf, Shizheng Wu and Yigang WangWen-Feng Cai, Wei Huang, Lei Wang, Jia-Peng Wang, Lu Zhang, Muhammad Ashraf, Shizheng Wu and Yigang Wang
  
  Background: Duchenne Muscular Dystrophy (DMD) is a recessive form of muscular disorder, resulting from the dystrophin gene mutations in X-chromosome. Application of embryonic stem cells or adult stem cells has demonstrated the therapeutic effects on DMD through both cell-based and non-cell based mechanisms. In this study, we proposed that Myogenic Progenitor Cells from Induced Pluripotent Stem Cells (iPSC-MPCs) would be more effective in repairing muscle damage caused by muscular dystrophy. Methods and results: Mouse iPSCs were cultured in myogenic differentiation culture medium and the MPCs were characterized using Reverse Transcription Polymerase Chain Reaction (RT-PCR) and flow cytometry. iPSCs were successfully converted into MPCs, as evidenced by the distinct expression of myogenic genes and cell surface markers. The muscle injury was induced in tibialis muscle of mdx mouse by card.. View More»
  DOI: 10.4172/2157-7633.1000361

  Abstract PDF