Journal of Microbial & Biochemical Technology

Koji Yamauchi

Publications

• Research Article
  Relationship between Bacterial Floras in Saliva and Tongue Coating and Volatile Sulfur Compounds in Oral Air
  
  Author(s): Kouichirou Shin, Izumi Aoyama, Koji Yamauchi, Fumiaki Abe and Ken YaegakiKouichirou Shin, Izumi Aoyama, Koji Yamauchi, Fumiaki Abe and Ken Yaegaki
  
  Volatile sulfur compounds produced by oral bacteria are known to be responsible for oral malodor. In this study, 20 volunteers without periodontitis were divided into malodor (n=10, H2S>1.5 ng/10 ml air or CH3SH>0.5 ng/10 ml air) and control (n=10) groups, based on a gas chromatography analysis of concentrations of volatile sulfur compounds in their mouth air. The total number of bacteria in whole saliva and tongue coating samples was analyzed by quantitative PCR, and the relative abundance of bacterial species was determined by targeted pyrosequencing of DNA encoding the V5-6 hypervariable region of 16S rRNA. The total number of bacteria in tongue coating suspensions analyzed by quantitative PCR was significantly higher in the malodor group compared to the control group. Total reads of 15,581 and 298,079 were yielded by pyrosequencing of saliva and tongue coating samples, respe.. View More»
  DOI: 10.4172/1948-5948.1000117

  Abstract