Journal of Microbial & Biochemical Technology

Kazutaka Itoh

Publications

• Research Article
  Isolation of Antioxidant Compounds from Aspergillus Terreus LS01
  Author(s): Rizna Triana Dewi, Sanro Tachibana, Kazutaka Itoh and Muhammad IlyasRizna Triana Dewi, Sanro Tachibana, Kazutaka Itoh and Muhammad Ilyas
  
  The antioxidative activity of an ethyl acetate extract from Aspergillus terreus LS01was evaluated using various in vitro assays: 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen-peroxide-scavenging free radical scavenging, and β-caroten-linoleat model assay and the antioxidants in the extract were isolated and identified. Silica gel column chromatography was used to separate the extract into five fractions (F1-F5). Fraction 3 possessed significant antioxidative activity with an IC 50 of 19.91μg/ml. Fraction 3 was further separated by column chromatography and yielded the crystalline compounds 1 and 2, which were identified as terreic acid and terremutin, respectively, on the basis of UV-vis spectra, MS and NMR analyses. Terreic acid and terremutin exhibited the highest level of DPPH free radical scavenging activity with IC 50 values of 0.115±4.02 and 0.114&plus.. View More»
  DOI: 10.4172/1948-5948.1000065

  Abstract PDF