 
        
						João Lúcio  Azevedo
	
					    												
Brazil						                            
                            
						
 Research Article
												Endophytic Bacteria Associated to Sharpshooters (Hemiptera:
Cicadellidae), Insect Vectors of Xylella fastidiosa Subsp. pauca 						
Author(s): Cláudia  Santos Gai, Francisco  Dini-Andreote1, Fernando Dini  Andreote, João Roberto  Spotti Lopes, Thomas Albert  Miller, João Lúcio  Azevedo and Paulo Teixeira  LacavaCláudia  Santos Gai, Francisco  Dini-Andreote1, Fernando Dini  Andreote, João Roberto  Spotti Lopes, Thomas Albert  Miller, João Lúcio  Azevedo and Paulo Teixeira  Lacava             
						
												
				 Xylella fastidiosa subsp. pauca causes citrus variegated chlorosis (CVC) disease in Brazil, resulting in significant production losses in the citrus industry. X. fastidiosa subsp. pauca is mainly transmitted by three species of sharpshooters (Hemiptera: Cicadellidae) in Brazil; Dilobopterus costalimai (Young), Acrogonia citrina Marucci & Cavichioli and Oncometopia facialis (Signoret). We identified bacterial communities associated with the heads of surface-sterilized insect vectors of X. fastidiosa subsp. pauca that were collected from CVC affected citrus groves in Brazil. Bacteria were isolated and analyzed by amplified ribosomal DNA restriction analysis (ARDRA) and sequencing, revealing the presence, among the most abundant genera, of the well-known citrus endophytes Methylobacterium spp. and Curtobacterium spp. Specific PCR systems for the detection of these genera indicated hi.. View More»
				  
												DOI:
												 10.4172/2157-7471.1000109