Journal of Allergy & Therapy

Hanisom Abdullah

Publications

• Research Article
  Production of Allergen Extracts for Bivalve Allergy Diagnosis Using Skin Prick Test
  Author(s): Zailatul Hani Mohamad Yadzir, Rosmilah Misnan, Faizal Bakhtiar, Noormalin Abdullah, Hanisom Abdullah and Shahnaz MuradZailatul Hani Mohamad Yadzir, Rosmilah Misnan, Faizal Bakhtiar, Noormalin Abdullah, Hanisom Abdullah and Shahnaz Murad
  
  Background: Whilst the consumption of bivalves is reasonably high in Malaysia, the frequency of allergy to this group of shellfish in the local population is largely unknown. The aim of this preliminary study was to produce bivalve allergen extracts and to investigate the frequency of bivalve sensitization among the local atopic population. Methods: Raw allergen extracts were prepared from 5 different species of bivalves. Their protein profiles were studied using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Results: In SDS-PAGE, the bivalve extracts demonstrated 10 to 23 protein bands. The five protein profiles varied considerably but most visible protein bands lay within 25-100 kDa. At the same time, fifty patients with a history of atopy were skin prick tested (SPT) with these raw extracts of bivalves. Of the 50 subjects, 13 (26%) had a positive SPT to at le.. View More»
  DOI: 10.4172/2155-6121.1000216

  Abstract PDF