Journal of Stem Cell Research & Therapy

Hanayuki Okura

Publications

• Research Article
  Spermine Treated-Adipose Tissue-Derived Multi-Lineage Progenitor Cells Improve Left Ventricular Dysfunction in a Swine Model of Chronic Myocardial Infarction
  Author(s): Hanayuki Okura, Mitsuko Morita, Maiko q Fujita, Kyoko Naba, Nozomi Hasebe-Takada, Akihiro Ichinose and Akifumi MatsuyamaHanayuki Okura, Mitsuko Morita, Maiko q Fujita, Kyoko Naba, Nozomi Hasebe-Takada, Akihiro Ichinose and Akifumi Matsuyama
  
  Background: The polyamine spermine enhances differentiation of mouse embryonic stem cells into cardiac lineage. The aim of this study was to determine the effects of spermine on the differentiation of human adipose tissuederived multi-lineage progenitor cells (hADMPCs) into cardiomyocytes both in vitro and in vivo and any subsequent functional effect in a swine model of chronic myocardial infarction. Methods and results: Spermine increased the expression of cardiac markers nkx2.5, islet-1, α-cardiac actin and cardiac troponin I (to 11.2-, 27.5-, 43.6- and 19.1-fold, relative to baseline, respectively) in hADMPCs. Chronic myocardial infarction model with left ventricular dysfunction was induced by balloon occlusion of the diagonal coronary artery followed by reperfusion, with subsequent similar procedur.. View More»
  DOI: 10.4172/2157-7633.1000326

  Abstract PDF