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Farhad Razjou
Farhad Razjou
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine,
IBTO, Tehran
West Indies
Publications
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Research Article
Measurement of BacT/ALERT Sensitivity after Inoculation Certain Amount of E. coli and S. epidermis
Author(s): Abolfazl Dabirmoghadam, Farhad Razjou and Bahram A. BadlouAbolfazl Dabirmoghadam, Farhad Razjou and Bahram A. Badlou
Background: Transfusion of contaminated human platelets concentrates (PCs) causes septic reaction and death of recipients. Current rate of detection of bacterial-contaminated of the PCs are very important tools to warranty patients’safety. BacT/ALERT system was introduced as one of the most sensitive, specific, and rapid screening test, which could be implemented in Blood Transfusion Centers. The aim of this study is to investigate whether the BacT/ALERT system as introduced, can function as rapid detection system. Material and Methods: In this study we used the whole blood from 24 healthy human subjects with inform consent, The PCs were collected from these whole blood samples by differential Centrifugation and Buffy coat system. All PCs (50-70 mL) stored under standard blood banking conditions. The PCs were randomly selected and divided in two groups of 12, and 10 CFU/.. View More»
DOI: 10.4172/2155-9864.1000268