Journal of Stem Cell Research & Therapy

Erika Fleck

Publications

• Research Article
  Mice Deficient in Rap1A GTPase Display Reduced Adhesion of Hematopoietic and Mesenchymal Stromal Cells but Intact Hematopoietic Repopulation Potential
  Author(s): Erika Fleck, Stephan Göttig, Harald Kropshofer, Juliane Steinmann, Annette Dorn, Brigitte Rüster, Erhard Seifried, Jürgen Scheele and Reinhard HenschlerErika Fleck, Stephan Göttig, Harald Kropshofer, Juliane Steinmann, Annette Dorn, Brigitte Rüster, Erhard Seifried, Jürgen Scheele and Reinhard Henschler
  
  Background: The Guanine Triphosphatase (GTPase) Rap1A has been implicated in cell proliferation and migration in different tissues. Its role in the production, adhesion and migration of bone marrow-derived hematopoietic cells is however incompletely understood. Methods: We analysed mice lacking one or both Rap1A alleles and measured their adhesion function and their hematopoietic repopulation capacity using competitive bone marrow transplantation in the CD45.1/CD45.2 transplant model. We further employed flow cytometry and shear stress-dependent flow chamber adhesion assays for phenotypic characterization and analysis of adhesion functions. Results: Rap1A-/- mice showed decreased numbers of lymphocytes and hematopoietic colony forming progenitors in the blood, while the numbers of granulocytes, monocytes or platelets were not significantly altered compared to controls. Chemokine CXCL12-i.. View More»
  DOI: 10.4172/2157-7633.1000356

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