Journal of Stem Cell Research & Therapy

Dygai AM

Publications

• Research Article
  Modulation Of Stem And Progenitor Cells, And Bleomycin-induced Pulmonary Fibrosis By Spiperone In Mice
  Author(s): Skurikhin EG, Pershina OV, Khmelevskaya ES, Ermakova NN, Reztsova AM, Krupin VA and Dygai AMSkurikhin EG, Pershina OV, Khmelevskaya ES, Ermakova NN, Reztsova AM, Krupin VA and Dygai AM
  
  Bleomycin-induced lung inflammation and fibrosis were assessed in a model of idiopathic pulmonary fibrosis in C57BL/6 mice and effect of spiperone on histopathological lung indicators, hematopoietic (HSC) and mesenchymal (MSC) stem cells and progenitor cells was characterized. Spiperone is a selective antagonist of D2 dopamine receptors which disturbs dopamine mediation. Hematoxylin and eosin staining showed spiperone decreased alveolar epithelial edema, exudation and infiltration of the alveoli walls and lumen by inflammatory cells (neutrophils, macrophages, plasma cells) after bleomycin instillation. Picrofuchsin staining by Van Gieson revealed spiperone decreases the area of connective tissue in the lung fibrotic phase of pulmonary fibrosis. ELISA assay determined the decrease levels of collagen type I, hydroxyproline and total collagen in lung homogenate after spi.. View More»
  DOI: 10.4172/2157-7633.1000210

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