Journal of Microbial & Biochemical Technology

Dr. Pillai Meena M

Publications

• Research Article
  Purification and Characterization of Superoxide Dismutase Isolated From Sewage Isolated E. coli
  Author(s): Petkar Medha B, Dr. Pillai Meena M, Kulkarni Amarja A, Bondre Sushma H and Dr KRSS RaoPetkar Medha B, Dr. Pillai Meena M, Kulkarni Amarja A, Bondre Sushma H and Dr KRSS Rao
  
  Superoxide dismutase (SOD), which plays a very important role in protecting organisms from oxygen toxicity, has therapeutic importance. It was purified from sewage isolated E. coli and characterized. Eukaryotic cells also produce SOD but culturing and maintenance of eukaryotic cells for production of SOD is costly as well as difficult. Using prokaryotic cells i.e. bacteria, production cost can be reduced. A rich bacterial source was identified. Bacterial membrane was ruptured in the presence of lysozyme and glass bead. Following ammonium sulphate precipitation, SOD-containing solution was applied to DEAE-cellulose and then Sephadex G- 75 gel columns. SOD was purified 63.91-fold with a specific activity of 3835U/ mg. The molecular weight was estimated to be 35.713 kDa by SDSPAGE gel. Maximum SOD activity was observed between pH 7.0 to 7.5 at temperature range 37–50ºC. This e.. View More»
  DOI: 10.4172/1948-5948.1000109

  Abstract PDF