Journal of Microbial & Biochemical Technology

David J. Geveke

Publications

• Research Article
  Effect of Thermal and Radio Frequency Electric Fields Treatments on Escherichia coli bacteria in Apple Juice
  Author(s): Dike O. Ukuku, David J. Geveke and Peter H. CookeDike O. Ukuku, David J. Geveke and Peter H. Cooke
  
  The need for a nonthermal intervention technology that can achieve microbial safety without altering nutritional quality of liquid foods led to the development of the radio frequency electric fields (RFEF) process. However, insight into the mechanism of bacterial inactivation by this technology is limited. In this study, we investigated membrane damage of Escherichia coli bacterial (7.8 log CFU/ml) and leakage of intracellular membrane materials in RFEF treated apple juice at 25 kV/cm and operated at 25°C, 55°C and 75°C for 3.4 milliseconds at a flow rate of 540 ml/min. Damage to cell membrane was detected with Transmission Electron Microscopy (TEM) and leakage of cellular materials was determined with ATP luminometer (20 D) and electrostatic and hydrophobic interaction chromatography used to characterize changes in bacterial cell surfaces. RFEF treatment caused a signific.. View More»
  DOI: 10.4172/1948-5948.1000074

  Abstract PDF