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Cynthia de Vries
Cynthia de Vries
Belgium
Publications
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Research Article
Guidelines to Optimize Survival and Migration Capacities of Equine Mesenchymal Stem Cells
Author(s): Sarah Broeckx, Cynthia de Vries, Marc Suls, Deborah J Guest and Jan H SpaasSarah Broeckx, Cynthia de Vries, Marc Suls, Deborah J Guest and Jan H Spaas
Background: Although mesenchymal stem cell (MSC) therapy is commonly being used in veterinary medicine, no specific guidelines are described to guarantee an optimal cell survival and migration. However, this is indispensable in order to assure an optimal clinical use. Methods: In the present study, equine peripheral blood (PB)-derived MSCs were isolated and characterized. In order to determine the optimal parameters for long-term cryopreservation, the effects of different dimethyl sulfoxide (DMSO) concentrations (5-20%) and thawing methods (temperature-controlled or hand-thawed) on MSC viability were evaluated by means of trypan blue staining. Afterwards, MSC storage was evaluated for 12 months using only 10% of DMSO as a cryoprotectant without rate-controlled freezing. Then, the influence of frequently used anaesthetics and injectable gels on MSC via.. View More»
DOI: 10.4172/2157-7633.1000147