Modern Chemistry & Applications

Costel C Darie

Publications

• Review Article
  Advances in Mass Spectrometry for Glycoscreening and Sequencing in Biomedical Research
  Author(s): Adrian Robu, Loredana Lupu, Roshanak Aslebagh, Alina D Zamfir and Costel C DarieAdrian Robu, Loredana Lupu, Roshanak Aslebagh, Alina D Zamfir and Costel C Darie
  
  During the past several years, applications of Mass Spectrometry (MS) in the biomedical research increased considerably. While MS is for many years heavily used in proteomics, for protein identification and quantification as well as for biomarker discovery, for a long time its applications in glycomics were limited, mainly because of the challenging conditions required for the ionization and detection of most carbohydrate classes. However, due to the development of high performance analytical instrumentation, MS in particular with Electrospray (ESI) and Matrix Assisted Laser Desorption/Ionization (MALDI) started to be intensively applied also to the analysis of post-translational modifications such as glycosylation, acetylation or phosphorylation. Focus on MS-based glycosylation is, however, scarce. Therefore, analysis of glycoproteins in particular diseases through glycoscreening and.. View More»
  DOI: 10.4172/2329-6798.1000138

  Abstract PDF