Journal of Microbial & Biochemical Technology

Carlos Roberto Félix

Publications

• Research Article
  Trichoderma reesei Mycoparasitism against Pythium ultimum is coordinated by G-alpha Protein GNA1 Signaling
  Author(s): Valdirene Neves Monteiro, Andrei Stecca Steindorff, Fausto Bruno dos Reis Almeida, Fabyano Alvares Cardoso Lopes, Cirano JoséUlhoa, Carlos Roberto Félix and Roberto Nascimento Silva Valdirene Neves Monteiro, Andrei Stecca Steindorff, Fausto Bruno dos Reis Almeida, Fabyano Alvares Cardoso Lopes, Cirano JoséUlhoa, Carlos Roberto Félix and Roberto Nascimento Silva
  
  Trichoderma reesei (Hypocrea jecorina) is widely explored in industry and its potential for using in agriculture as a biocontrol agent against phytopathogenic fungi has just began to be investigated. We have investigated the involvement of G proteins during mycoparasitism against plant pathogens. Here we described the role of GNA1, a G-alpha protein that belongs to αi group in Cell Wall Degrading Enzymes (CWDEs) production by T. reesei during antagonism against Pythium ultimum. For that, two mutants were used: Δgna1 and gna1QL (=constitutively activated version of GNA1). The gna1QL mutant of T. reesei, like the parental TU-6, inhibited the growth of P. ultimum in plate confrontation assay and grew faster than the parental TU-6 while the Δgna1 did not grow over P. ultimum. Scanning electron microscopy showed that the gna1QL mutant promoted more morphological alteratio.. View More»
  DOI: 10.4172/1948-5948.1000173

  Abstract PDF