Aymen S Yassin
Egypt
Research Article
Screening of Bio-Surfactant Production Ability among Organic Pollutants
Degrading Isolates Collected From Egyptian Environment
Author(s): Mariam Hassan, Tamer Essam, Aymen S Yassin and Aisha SalamaMariam Hassan, Tamer Essam, Aymen S Yassin and Aisha Salama
A total of ten bacterial isolates were screened for their biodegradation, metabolic versatility and biosurfactants production using various organic pollutants. The biosurfactants production ability was mainly assessed by oil spread test (OST) and/or emulsification assay (EA). Although initial biosurfactants screening was conducted using paraffin oil, the application of vegetable oils, particularly coconut oil, was always accompanied with the highest yield of biosurfactants production,. Biochemical and molecular identification of the ten isolates revealed that they belong to three genera; Klebsiella (6), Pseudomonas (3) and Citrobacter (1). Interestingly, four isolates (M2H2 1, M2H2 3, M2H2 8 and M2H2 14), showed the highest biosurfactants production and therefore were further assessed using mixed carbon source (coconut oil in combination with one organic pollutant (phenol or cyclohexa.. View More»
DOI:
10.4172/1948-5948.1000143