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Allenspach K
Allenspach K
Tanzania
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Research Article
Effects of Different Oligosaccharides on Growth of Selected Probiotic Bacterial Strains
Author(s): Salavati Schmitz S and Allenspach KSalavati Schmitz S and Allenspach K
Abstract Objective: To assess if prebiotics at different concentrations can accelerate the growth of selected probiotic bacterial strains. Materials and methods: Enterococcus (E.) faecium NCIMB 10415 E1707 was chosen as it is the most common probiotic strain used for small animals. In addition, E. faecium NCIMB 30183, Bifidobacterium (B.) longum NCIMB 30182 and B. infantis NCIMB 30181 were tested. They were grown in 96-well plates and growth was assessed by optic density at 600 nm, using a bacterial plate reader. The prebiotics used were Fructo-Oligosaccharides (FOS), mannan oligosaccharides (MOS) and Preplex® (a combination of FOS and gum Arabic available in a commercial synbiotic product for small animals). Initially, addition of inulin was also planned but not achieved due to technical difficulties. The prebiotics were used at 20 mg/ml, 10 mg/ml, 1 mg/ml and 0.1 mg/ml, respec.. View More»
DOI: 10.4172/1948-5948.1000343