The present study aims to study the effect of Vitamin D (Vit D) or/and Coconut Oil (Coc) on the splenic histological changes of diabetic adult mice induced by STZ. The mice were divided into 7 groups, and the experimental duration was 4 weeks. Group I: Control group without any treatments; group II and group III: Non-diabetic groups orally received vitamin D in a dose of 500 IU (6.25 ml)/kg bw/d or Coc in a dose of 7.5 ml/kg bw/d; group IV: Diabetic group
injected IP with a single dose of STZ (200 mg/kg bw); groups V, VI and VII: Administration of vitamin D or Coc or both together to diabetic group. The results recorded: Group II and group III no significant changes in the Blood Glucose (BG), insulin splenic weight levels. Group IV had a significant increase in BG, significant decrease in insulin and splenic weight values. Group V recorded a modest decrease in BG, and a modest rise in insulin andmarked splenic weight atrophy; while group VI or group VII registered a marked decrease in BG, a rise in insulin levels a marked splenic weight increment. Histologically, the splenic sections of control or non-diabetic mice received either vitamin D or Coc demonstrated normal structure of the solenocytes. Group IV showed great numbers of giant cells, disarrangement and interference of red and white pulps, and dilated congested blood vessels. Little improvement was seen in group V while group VI or group VII showed a marked improvement in the splenic tissues. In conclusion, diabetic mice received either Coc or co-dministered with vitamin D demonstrated strong anti-hyperglycemic effects to recovery the glucose and insulin rates to typical levels, and restored the splenic weight and histological architecture to normal status than those given vitamin D alone.
Published Date: 2023-05-29; Received Date: 2023-03-11