Journal of Stem Cell Research & Therapy

Abstract

Osteogenic Differentiation of Human Umbilical Cord Peri-vascular Cells using Low Intensity Pulsed Ultrasound

Taghreed A Aldosary, Hasan Uludag, Michael Doschak and Tarek El-Bialy

The objective of this experiment was to explore the possible effect of low intensity pulsed ultrasound (LIPUS) on the osteogenic differentiation of harvested passage-4 Human umbilical cord peri-vascular cells (HUCPV-Cs). HUCPV-Cs were divided into two groups: a treatment group that received LIPUS for 10 minutes for 1, 7 and 14 days and a control group that received a sham treatment utilizing osteogenic media. The results demonstrated nonsignificant differences in cell count, ALP, DNA content, and CD90. Statistically significant expression of OPN and PCNA was observed on day 14 in the LIPUS treated group. Nucleostemin expression in the LIPUS-treated group was nonsignificant on days 1 and 7. However, a selective increase in the osteogenic markers was observed in the LIPUS treated group on day 7 for ALP and OCN and on day 14 for OPN. Future experiments are required to explore the possible effects of different application times and/or techniques of LIPUS on the behaviour of HUCPV-Cs.