Journal of Nanomedicine & Nanotechnology

Abstract

Green Synthesis of Copper-Chitosan Nanoparticles and Study of its Antibacterial Activity

Appu Manikandan and Muthukrishnan Sathiyabama

Copper nanoparticle (Cu-NP) synthesis has been gaining attention due to its property and applicability. The main objective of the work is to synthesize stable Cu-NP through green synthesis. This work demonstrates the synthesis of Cu-NP by the addition of the acidic chitosan solution to CuSO₄ solution with constant stirring at 70°C for 12 h. The addition of chitosan aids the stable formation of nanoparticle. The formation of nanoparticle was observed by the peak at 536 nm using UV-Vis spectroscopy. The interaction between the chitosan and the synthesized nanoparticles was studied using FTIR spectroscopy. The average size of the nanoparticle as determined by zetasizer was about 88.21 nm, while the zeta potential was -29.0 mV. Transmission electron microscopy (TEM) analysis indicated the spherical size with a size ranging between 20-30 nm. The Energy-dispersive X-ray spectroscopy (EDS) analysis showed a homogenous copper-rich composition of metal nanoparticles. The X-ray diffraction (XRD) analysis confirmed the presence of copper nanoparticle with crystalline nature. The synthesised Cu-chitosan nanoparticle exhibited antibacterial activity against gram negative as well as gram positive bacteria. However, the activity was more against gram negative bacteria which may be due to the difference in cell wall composition.