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Abstract
Expression of some genes involved in epigenetic in breast cancer cell line (MCF7): The effect of gold nanoparticles
Mojgan Noroozi-Karimabad*, Marzie Salandari Rabori, Omid Azizian-Shermeh and Seyedeh Atekeh Torabizadeh
Background: Nano medicine is defined as the utilizing nanoparticles for treatment and diagnosis of diseases. Gold nanoparticles to be surveyed. Green synthesis is another alternative as an economically viable, simple, and environmentally friendly method for synthesizing gold nanoparticles. Here we have investigated the potential of gold nanoparticles synthesis in inhibiting the expression of self-renewal regulatory factors and cancer stem cell gene and apoptosis genes in a leukemia cell line MCF7.
Methods: Using green chemistry technique, gold nanoparticles were synthesized using walnut green external shell, where the green shell of walnut is a reducer and stabilizer for providing gold nanoparticles. These nanoparticles were examined using x-ray diffraction (XRD) and transmission electron microscopy (TEM) to determine their size, structure, and shape. Cell proliferation inhibition was tested using MTT assay. Real-time-PCR method was applied to indicate the fold changes of P53, NANOG, SOX, OCT4, and CAS expression against β-actin. Data were analyzed by Two-Way ANOVA Differences were considered significant if (P<0.05).
Result: A significant variation was observed in cell viability when different gold nanoparticles’ concentrations were applied for 24 h compared to control with regard to the cellular viability (P <0.05). Real time-PCR analysis showed that the NANOG, OCT4, and SOX gene expression levels down-regulated while CAS and P53 were up-regulated compared to untreated control cells and cells treated (P<0.05).
Conclusion: The results revealed that gold nanoparticles may hinder the proliferation of cancer in in vitro, resulting in the development of a desired treatment agent for treating MCF7 cancer. To sum up, the current study offers promising information on stem-cell genes expression in MCF7 cells.
Published Date: 2023-11-30; Received Date: 2023-11-01