Abstract

Control of Bacterial Blight Disease of Pomegranate Using Silver Nanoparticles

Sherkhane AS, Suryawanshi HH, Mundada PS and Shinde BP

Successful cultivation is difficult to pomegranate growers due to Xanthomonas axonopodis pv. punicae which cause bacterial blight disease to pomegranate has become serious threat. However, everyone is looking for solution; this problem is resolved by synthesizing silver nanoparticles by the reduction of silver ions in aqueous solutions with the help of green synthesis method. In the present study the causative agent was isolated from the infected pomegranate fruits and was tested with silver nanoparticles which are synthesized from leaf extracts of Tulsi, Neem, Tridax and Drumstick at variable concentrations and it was found that silver nanoparticle containing Tulsi extract with 15 mM concentration showed maximum zone of inhibition (22 mm), then Tridax (20 mm), Neem (19 mm) and Drumstick (18 mm) while at 1 mM concentration it shows minimum. The bioreduction was tested by using UV-Vis Spectrophotometer. This study showed that by using synthesized silver nanoparticles we successfully controlled the growth of Xanthomonas axonopodis pv. Punicae. They can be used in the field of medical as well as in the management of plant diseases