The plasma membrane of a mature red blood cell has glycol-proteins and glycol-lipids that determine a person’s blood type. Blood types A, B, AB, and O form the ABO blood group. ABO blood type is determined by the hereditary presence or absence of antigens A and B on Red Blood Cells. Blood types and transfusion compatibility are a matter of interactions between plasma proteins and erythrocytes. A blood sample was taken from the voluntary Physics students of Dilla University. Blood type (groups) varies among each ethnic group, A, B, AB and O blood groups were analysis for these ethnic group of Physics department students found in Dilla University, Amhara state, Oromia state, South Nation Nationalities People, Tigray state and Gembella people. The distribution of blood group O was the highest with percentage frequency of 38.33, 29.44 and 28.88%, followed by blood group A and blood group B, and the least percentage frequency is that of blood group AB with 3.33% among each ethnic groups. The highest Rh-blood types were 91.66% Rh-positive blood types followed by 8.34% Rh-negative blood types. Therefore, among each ethnic groups of Ethiopian students, the blood type and groups were varies in all voluntary students of Dilla University.