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Research Article - (2018) Volume 10, Issue 2

Effect of Storage Time on Nutrient, Biodeteriorating Fungi and Aflatoxin Contents of Bitter Leaf (Vernonia amygadalina) and JUTE (Corchorus olitorius)

Jonathan Segun G, Onile Olanrewaju G, Asemoloye Michael D* and Omotayo Omolola O
Department of Botany, Mycology/Biotechnology Unit, University of Ibadan, Nigeria
*Corresponding Author: Asemoloye Michael D, Department of Botany, Mycology/Biotechnology Unit, University of Ibadan, Nigeria, Tel: 2348064844977 Email:

Abstract

Unhygienic handlings of several African food products during storage have been reported to promote fungal biodeterioration and subsequent aflatoxin contamination. Therefore, this study was aimed to investigate the effect of storage time on nutrient, commonly associated bio-deteriorating fungi and aflatoxin contents of selected vegetables; Bitter leaf (Vernonia amygdalina) and Jute (Corchorus olitorius) in Nigeria. Fresh and stored vegetable samples (24) were collected from different markets in Ibadan, Nigeria, they were analyzed for proximate (% Moisture, Carbohydrate, Crude Protein, Fat and Fibre), nutrient (Potassium, Calcium, Phosphorus, Sodium Iron, Zinc and Magnesium), fungal and aflatoxin contents (AFB1, AFB2, AFG1 and AFG2). Most dominantly associated fungal strains were identified as Aspergillus niger, Aspergillus flavus, Aspergillus terreus, Aspergillus tamari, Aspergillus parasiticus, Aspergillus fumigatus, Penicillium chrysogenum and Rhizopus nigricans. The fungal incidence was more pronounced in the stored samples as compared to the fresh samples. The storage time also significantly affected the nutrient and aflatoxin contents of the vegetables; most nutrients decreased with increasing storage period while the aflatoxin contents increased. The study affirms that the storage time has effect on the nutrient, fungal contamination and consequently on the associated aflatoxin, although the detected aflatoxin levels were below the tolerance limit but these may increase with the storage time. It is therefore advisable to take hygienic measures during storage period and also, prolong storage should be discouraged.

Keywords: Unhygienic handling, Storage time, Fungal biodeterioration, Nutrient composition, Aflatoxins, Health implications

Introduction

Fungal contaminations of foods and feeds is currently raising global food safety concerns due to their ability to colonize food items, either causing physical damage or release some toxic secondary metabolites commonly known as ‘Mycotoxins’ [1]. The most commonly identified mycotoxin in African foods and feeds is ‘Aflatoxin (AF)’, AF is produced by fungi of the genera Aspergillus, there are four major aflatoxins; AFB1, AFB2, AFG1 and AFG2 due to their high prevalence in nature and toxicity [2]. AF has been established to be powerful carcinogenic, cytotoxic, mutagenic and teratogenic substances to human [3]. Most susceptible food stuffs for the aflatoxin contamination are corn, barley; wheat, coffee beans, peanuts, spices, herbs, some raw or processed fruits and vegetables. In Nigeria, Aflatoxins have been reported in yam chips, tomatoes and pawpaw [4-14]. However, there are limited reports on vegetables either in fresh or stored forms in Nigeria.

Vegetables are readily used either in fresh or dried forms, some dried leaves are preferably eaten in some parts of Nigeria on daily basis. Fungal spoilage of vegetables can occur during storage, handling and transportation as well as market and unfortunately, vegetable products that are susceptible to fungi growth can also be contaminated with aflatoxins [15,16]. Drying is usually the practice for storing vegetables in Nigeria to make the products more durable and the bulk of it preserved so they may be available all year round. However, unhygienic handling of dried vegetable products may still make them susceptible to infestation with fungi and other contaminants.

Bitter leaf (Vernonia amygdalina) for example is a green shrub in family Asteraceae; it has petiole leaf of about 6mm diameter and characteristic odor and bitter taste. Vernonia amgydalina grows under a range of ecological zones in Africa being drought tolerant and produces large fodder biomass for both human and animal nutrition [17,18]. Vernonia amygdalina plant is commonly found around homes in Southern Nigeria, it is commonly used as a green vegetable or spice especially in the popular ‘bitter-leaf soup’ also, it is widely used for both therapeutic and nutritional purposes [19]. Also, another common vegetable Nigeria is Jute (Corchorus olitorius L.); it belongs to the family Tiliaceae. Jute is a fiber crop and one of the popular tropical leafy vegetable in Africa, Asia and some parts of the Middle East and Latin America [20]. It is a common leafy vegetable in Cote d’Voire, Benin, Nigeria, Cameroon, Sudan, Kenya, Uganda and Zimbabwe [21]. It is popularly known as Ewedu in the Southwestern part of Nigeria, where the boiled and mashed fresh leaf is a delicacy and the mucilaginous leaves are consumed along with other staples. It is extremely consumed as a health vegetable, because it contains abundant β-carotene and other carotenoids, vitamins B1, B2, C and E, and minerals. The vegetable also has varying proportion of dietary fibre and protein required for health [22].

This research was therefore embarked upon to investigate the effect of storage time on the nutrient, associated biodeteriorating fungi and aflatoxin contents of fresh and dried Verlonia and jute vegetables.

Materials and Methods

Collection of samples

Freshly harvested healthy samples of Bitter leaf (Vernonia amygdalina) and Jute (Corchorus olitorius) were purchased in bulk from different markets in Nigeria as shown below:

i. Bodija market; 7.43 51’N, 3. 91 43’E

ii. Moniya market; 7. 52 49’N 3.91 51’E

iii. Oja Oba market; 7. 37 39’N, 3. 57 20’E

They were collected in sterile polythene bags and labeled according to time and location; they were then taken to the Pathology/Mycology Laboratory of Department of Botany, University of Ibadan for further studies.

Sample preparation

The leaf samples were removed from their stalks, sliced and divided into two halves. One half was air dried, labeled and stored for respective months till when desired. The other half was immediately analyzed for fungal isolation, nutrient and aflatoxin compositions.

Isolation and characterization of fungi biota

The isolation of fungi was carried out according to procedure described by all samples collected were conditioned in a sterile package [23]. About two grams of each of the samples taken at random were aseptically placed in three replicates of Petri-dishes containing Potato Dextrose Agar and Lactic acid (10.53 g mL-1). The dishes were incubated at 27 ± 2°C for 3-6 days. Fungal cultures obtained were subsequently sub-cultured for purification. Upon fungi maturation, they were characterized based on cultural and morphological features such as colony diameter, colony color on agar, front and reverse and colony texture. Slide culture was then prepared and incubation in moist chambers at 26 ± 2°C for 4 days before observation under a light microscope (labomed, model CxL). Mycological keys and manuals were used for macro and microscopic features that are commonly used identification of fungi, which were conidiophores, conidial shape, phialides and metulae, presence and shape of vesicles [24].

Aflatoxin extraction, detection and quantification

Extraction: 1 g (of each sample) was pipetted into a 100 mL conical flask. 2.5 mL of distilled and 25 mL of chloroform water were added. The flask was covered with a stopper and shake in a shaker for 30 min after which the solution obtained was filter using a Whattman no. 1 filter paper. 10 mL of each extract or filtrate was collected and evaporated to dryness to a volume of 5 mL on a hot water bath. 5 mL extract was stored in dark bottles in a freezer for detection and quantification [25].

Detection of aflatoxin: 1 mL chloroform and 0.2 mL of the reconstituted extract was spotted on a precoated 20 × 20 cm TLC plate along with aflatoxin standards of known concentration. The spotted TLC plate was developed in an equilibrated tank containing chloroform: acetone (9:1 v/v). The developed TLC plate was air-dried at ambient temperature (28 ± 2°C) and aflatoxins were detected under UV light at wavelength of 360 nm. A colour change from blue to yellow upon exposure to aqueous sulphuric acid (50:50 v/v) confirmed the presence of Aflatoxin B1. Aflatoxin B2 was derived from Aflatoxin B1 as dihydroderivative which experienced a colour change from pale blue to deep yellowish color upon exposure to aqueous sulphuric acid (50:50) to confirm its presence. Aflatoxin G1 fluoresced yellowish green upon exposure to UV light while Aflatoxin G2 fluoresced pale yellowish green upon exposure to same UV light [25].

Quantification of aflatoxin: 0.5 μm thick preparative TLC plates was employed for the quantitation of stored extract after aflatoxin extraction was applied to the plate as band rather than a spot to chromatograph, the maximum amount of sample at the same time. The preparative TLC plates were developed in an equilibrated tank as an aflatoxin extraction. When the solvent front had risen to about ¾ of the total length of the plate, the plate was taken out of the tank and examined under UV light. The area containing the toxin of interest was located and scrapped off, elute with chloroform and filter using Whattman No. 1 filter paper. The extract was evaporated to dryness over a hot water bath and reconstituted with 3 mL chloroform. The 3 mL reconstituted solution and aflatoxin standard of 20 μg/mL concentration was used to read Absorbance or Optical Density on an ultraviolet Spectrophotometer (Cecil Instrument CE505) at a wavelength of 360 nm [25]. Aflatoxin concentration in μg/kg was calculated using the formula:

equation

Crude protein determination: The crude protein in the samples was determined by the routine semi-micro Kjeldahl, procedure. 0.5 g of each finely ground dried sample was weighed carefully into the Kjeldahl digestion tubes, 1 tablet of selenium catalyst and 10 mL of conc. H2SO4 was added into the digestion preset at 500°C. The digestion was left for 4 h in a fume cupboard. The tube was placed in a distilling unit and 5 mL of 40% NaOH was added to it. The mixture was steam distilled for 2 min into a 50 mL flask containing 10 mL of 2% Boric acid, mixed with indicator solution which was then titrated against 0.01 N HCl until a wine colour was obtained. The quantification was done using AOAC procedure [26].

Crude fat determination: 250 cm3 clean boiling flasks were dried in oven at 105-110°C for about 30 min. It was later transferred into desiccator and allowed to cool. About 2 g of sample was weighed accurately into labeled thimbles. The correspondingly labeled cooled boiling flasks were weighed. The boiling flasks were filled with about 300 cm3 of petroleum ether (boiling point 40°C-60°C). The extraction thimble was plugged with cotton wool. The soxhlet apparatus was assembled and allowed to reflux for about 6 h. The thimble was removed with care and the petroleum ether was collected in the top of the container of the set-up and drained into a container for re-use. When the flask was almost free of petroleum ether it was removed and dried at 105°C-110°C for 1 h. It was later transferred into a desiccator from the oven and allowed to cool and weighed [26].

equation

Dry matter and moisture determination: 2 g of the sample were weighed into a previously weighed crucible (W0). The crucible plus sample (W1) taken was then transferred into the oven set at 100°C to dry to a constant weight for 2 h. At the end, the crucible plus sample was removed from the oven and transferred to desiccator, cooled for 10 min and weighed (W3) [26].

equation

Ash determination: 2 g of each sample was weighed into a porcelain crucible. This was transferred into the muffle furnace set at 550°C and left for about 4 h. About this time it had turned to white ash. The crucible and its content were cooled to about 100°C in air, then room temperature in a desiccator and weighed [26].

equation

Fibre determination: 2 g of the sample was weighed accurately into the fibre flask and 100 mL of 0.255 N H2SO4 was added. The mixture was heated under reflux for 1 h with the heating mantle. The hot mixture was filtered through a fibre sieve cloth. The residue was returned to the fibre flask to which 100 mL of (0.313 N NaOH) was added and heated under reflux for another 1 h. The mixture was filtered through a fibre sieve cloth and 10 mL of acetone added. The residue was washed with about 50 mL hot water on the sieve cloth before it was finally transferred into the crucible. The crucible and the residue were oven-dried at 105°C overnight to drive off moisture. The oven-dried crucible containing the residue was cooled in a desiccator and weighed to obtain the weight W1. The crucible with weight W1 was transferred to the muffle furnace for ashing at 550°C for 4 h. The crucible containing white or grey ash was cooled in the desiccator and weight to obtain W2. The difference W1 – W2 gives the weight of fibre [26].

equation

Carbohydrate (CHO): The carbohydrate content was determined by using the formula:

(Moisture content + Crude fat + Crude Protein + Crude Fibre + Ash) – 100 [26].

Phosphorus, sodium, potassium, calcium, copper, zinc, magnesium and iron determination: Phosphorus was determined routinely by the Vanado-Molybdate Colori-Metric method, 0.2 g of well ground samples of both Mallow leaf and Bitter leaf were weighed into a dried crucible and Put inside a furnace set of 600°C and allowed the sample to ash for 2 h. The ash sample was washed by pipetting 10 mL of INHCL and placed on a hot plate to evaporated to dryness, then another 10 mL of HCL was added and it was been removed from hot plate. It was allowed to cool and washed into a 10 mL volumetric flask using filter paper and funnel and it was made with distilled water H2O up to 60-100 mL, into a 50 mL volumetric flask, pipette 10 mL from 100 mL of vanadate yellow to it and made it up with distilled H2O, allowed it to develop for 15 min. The spectrometer was set at 470 mm and allowed to warm for 15 min and the standard phosphorus was prepared and read first before the sample [26].

The percentage phosphorus was then calculated from this formula:

PPm=Meter reading × Average gradient × 1st dilute × 2nd

Dilution factor divided by weight of sample × 1

Sodium, potassium and calcium: From the washed sample (i.e., 100 mL) flame photometer was used to read the level of Na, K Ca, after standardized it with is respective minerals. The percentage individual elements were carried out using the formula.

4.4.12 PPM=Copper, zinc and iron: Dilution 1:25 were made for mg level determination from the washed sample, i.e., 100 mL flask, after the dilution, it was then read on Atomic Absorption Spectrometer (AAS), after standardized it with Mg standard. Cu, Zn, Fe were read from the solution that remains in the 100 mL flask and also read on AAS after standardized with respective elements [26].

They were calculated in parts per million (PPM) by the formula:

equation

Statistical analysis of data

The data collected were categorized and analyzed using CoStat 9.0 version and the homogeneity of means was done using Duncan Multiple Range Test (DMRT) at 95% confidence level (Figure 1).

Figure

Figure 1: Data were represented on tables as means ± Standard deviation, while qualitative data were described using figures and charts.

Results

Incidence of biodeteriorating fungi associated with the vegetables samples

The fungal strains that most commonly isolated from the samples are Aspergillus niger, A. flavus and Rhizopus nigricans with the highest percentage incidences while A. terrus, Penicillium oxalicum, A. tamari, A. fumigatus while A. parasiticus, fusarium oxysporum, penicillium chrysogenum and Trichoderma sp. had the least incidence (Table 1). It was observed that the dominant fungal are those regarded as aflatoxigenic fungal strains due to their ability to produce aflatoxins. Furthermore, the fungi incidence was also observed to be influenced by the sample location, Bodija samples had the most fungi incidence followed by Oja Oba and Moniya samples.

  Vernonia amygdalina Corchorus olitorius
Biodeteriorating fungi Bodija Oja Oba  Moniya Total
Occurrence
% Incidence Bodija Oja Oba  Moniya Total Occurrence % Incidence
Fusarium compacticum 0 0 0 0 0 0 0 0 0 0
F. oxysporum     0 0 1 0 2.2 1 2 1 4 8.7
Penicillium oxalicum     2 1 3 6 13 1 2 3 6 13
Penicillium chrysogenum     0 1 0 1 2.2 1 0 1 1 2.2
Rhizopus nigricans     3 3 1 7 15.2 3 2 2 7 15.2
Trichoderma spp.        1 0 0 1 2.2 0 0 0 0 0
Aspergillus tamarii      0 1 1  2 4.3 2 1 0 3 6.5
Aspergillus terrus    3 3 1 7 10.9 3 1 0 4 8.7
Aspergillus niger       1 2 0 3 6.5 3 3 2 8 17.3
Aspergillus parasiticus       4 3 2 9 19.6 2 1 1 4 8.7
Aspergillus flavus      1 2 0 3 6.5 3 2 2 7 15.2
Aspergillus fumigatus   1 0 1 2 4.3 1 0 1 2 4.3
Total      20 14 12 46 100 20 14 12 46 100
                     

Values are means of tree replicates

Table 1: Percentage incidence of biodeteriorating fungi associated with the selected vegetables.

Proximate analysis of Vernonia amygdalina and Corchorus olitorius

The storage time and location had significant effect on the proximate compositions of the Vernonia amygdalina and Corchorus olitorius samples (Table 2). Apart from the moisture content, the vegetables generally had higher carbohydrate content, followed by crude protein, and fibre while cru fat is the least. The moisture content, crude protein, fibre, ash and fat contents of the vegetables significantly reduced at three month of storage across all the locations as compared to the fresh samples. However, the carbohydrate content of the samples increased at 3 months storage time (Table 2).

    Proximate Composition (%)    Fresh Samples       Three months stored samples       
Parameters   Sample       Bodija   Oja-Oba   Moniya  Bodija     Oja-Oba   Moniya
Crude Protein Vernonia amygdalina     21.4 ± 0.05c  22.8 ± 0.00b  23.6 ± 0.14a  18.5 ± 0.13f  19.4 ± 0.01e  20.5 ± 0.07d
  Corchorus olitorius    5.71 ± 0.01c 5.91 ± 0.01b 6.05 ± 0.07a 4.29 ± 0.02f 4.81 ± 0.01d 4.59 ± 0.02e
Crude Fibre Vernonia amygdalina 9.46 ± 0.08c 10.4 ± 0.00a 8.95 ± 0.01d 9.81 ± 0.01b 8.51 ± 0.01e 7.82 ± 0.03f
  Corchorus olitorius  1.54 ± 0.01a 1.50 ± 0.03a 1.48 ± 0.01b 1.34 ± 0.01c 1.30 ± 0.01d 1.11 ± 0.01e
Crude Fat  Vernonia amygdalina 0.11 ± 0.01f 8.90 ± 0.00b 9.79 ± 0.05a 0.17 ± 0.00e 7.11 ± 0.01d 8.60 ± 0.00c
  Corchorus olitorius  0.26 ± 0.01a 0.24 ± 0.01a 0.25 ± 0.01a 0.21 ± 0.01b 0.20 ± 0.00b 0.21 ± 0.01b
Ash Vernonia amygdalina 6.46 ± 0.02b 5.96 ± 0.00c 7.32 ± 0.25a 4.25 ± 0.01f 4.82 ± 0.02e 5.23 ± 0.02d
  Corchorus olitorius  0.13 ± 0.01b 0.15 ± 0.01a 0.14 ± 0.00a 0.10 ± 0.00d 0.14 ± 0.00a 0.11 ± 0.00c
Moisture Vernonia amygdalina 10.5 ± 0.14c  10.7 ± 0.00b 11.8 ± 0.10a 7.0 ± 0.00f  8.4 ± 0.00d  7.4 ± 0.00e
  Corchorus olitorius  10.9 ± 0.03b 11.2 ± 0.33a  10.6 ± 0.52c  7.0 ± 0.00f  7.5 ± 0.00e  8.3 ± 0.00d
Carbohydrate Vernonia amygdalina 62.5 ± 0.06b 52.1 ± 0.00e 50.4 ± 0.43f  69.3 ± 0.13a  60.2 ± 0.05c  57.9 ± 0.12d
  Corchorus olitorius  92.4 ± 0.03b  92.2 ± 0.06b 92.1 ± 0.10b  94.1 ± 0.04a  93.6 ± 0.01a 93.5 ± 0.66a

Values are means of tree replicates, values with the same letter in a column are not significantly different from each other at p>0.05

Table 2: The proximate analysis of vegetable samples.

Samples of Vernonia amygdalina and Corchorus olitorius collected from Moniya market, Ibadan had the highest crude protein contents of 23.6 ± 0.14% and 6.05 ± 0.07%, respectively while Oja-Oba market had the highest crude fibre content (10.4 ± 0.00% and 1.50 ± 0.03%, respectively. Crude fat content was detected highest in fresh Corchorus olitorius sample collected from Bodija (0.26 ± 0.01%), Oja-Oba (0.24 ± 0.01%) and Moniya (0.25 ± 0.01%) markets, while Vernonia amygdalina had the highest percentage crude fat in fresh Moniya sample (9.79 ± 0.05). Highest moisture content was recorded in stored Oja Oba bitter leaf samples (8.4 ± 0.00%) and dried Moniya Corchorus olitorius samples (8.3 ± 0.00%). The highest % carbohydrate composition was recorded for dried and stored Corchorus olitorius samples collected from Bodija (94.1 ± 0.04%), Oja- Oba (93.6 ± 0.01%) and Moniya (93.5 ± 0.66%) markets while the stored samples of Vernonia amygdalina collected from Bodija market had the highest composition of carbohydrate (69.3 ± 0.13%) followed by Oja Oba (60.2 ± 0.05%) and Moniya (57.9 ± 0.12%).

The mineral analysis of Vernonia amygdalina and Corchorus olitorius

The storage time and location also had significant effect on the mineral compositions of the Vernonia amygdalina and Corchorus olitorius samples Table 3. Generally, the vegetable samples are richer in Calcium, Potassium and Phosphorus while they contain lower amount of Sodium, Iron, Zinc and Magnesium. It was also noticed that the mineral compositions of the sample decreased at 3 months of storage.

                 Mineral Composition (mg/L)
       Fresh Vegetable Samples Three(3) Months Old Samples
Minerals Vegetables Bodija Oja-Oba Moniya Bodija Oja-Oba Moniya
Sodium Vernonia amygdalina 6.30 ± 0.71b 7.40 ± 0.00ab 7.93 ± 0.95a 4.71 ± 0.01c 6.71 ± 0.01ab 7.30 ± 0.14ab
Corchorus olitorius 10.95 ± 0.49a 11.1 ± 0.42a 11.59 ± 0.16a 8.69 ± 0.02c 8.30 ± 0.00c 9.69 ± 0.01b
Potassium Vernonia amygdalina 216.41 ± 0.01c 217.6 ± 0.00b 219.07 ± 0.52a 210.29 ± 0.02f 214.85 ± 0.07d 212.35 ± 0.35e
Corchorus olitorius 272.5 ± 1.27bc 275.77 ± 1.89a 274.02 ± 0.59ab 260.25 ± 0.21d 271.0 ± 0.85c 270.11 ± 0.01c
Calcium Vernonia amygdalina 252.15 ± 0.78a 248.1 ± 0.00b 252.05 ± 0.92a 248.5 ± 0.14b 244.8 ± 0.14c 247.25 ± 1.20b
Corchorus olitorius 265.2 ± 1.27b 261.2 ± 0.71c 267.23 ± 0.38a 258.6 ± 0.00d 251.2 ± 0.57d 258.45 ± 0.07e
Phosphorus Vernonia amygdalina 220.9 ± 0.28a 216.7 ± 0.00c 216.91 ± 0.01c 218.55 ± 0.07b 210.15 ± 0.07e 211.65 ± 0.07d
Corchorus olitorius 123.1 ± 0.42e 126.85 ± 1.06c 262.35 ± 0.78a 120.55 ± 0.07f 124.61 ± 0.01d 259.33 ± 0.52b
Iron Vernonia amygdalina 3.55 ± 0.01c 3.72 ± 0.00b 3.84 ± 0.04a 2.71 ± 0.01e 2.85 ± 0.01d 2.73 ± 0.01e
Corchorus olitorius 7.18 ± 0.11b 7.73 ± 0.01a 7.63 ± 0.24a 5.85 ± 0.01e 6.11 ± 0.01d 6.43 ± 0.02c
Zinc Vernonia amygdalina 0.82 ± 0.01e 1.44 ± 0.00c 1.74 ± 0.01a 0.77 ± 0.00f 1.31 ± 0.01d 1.60 ± 0.00b
Corchorus olitorius 4.76 ± 0.06b 5.16 ± 0.08ab 5.52 ± 0.40a 3.74 ± 0.02d 3.83 ± 0.01cd 4.20 ± 0.00c
Magnesium Vernonia amygdalina 4.63 ± 0.01a 4.57 ± 0.00b 4.66 ± 0.01a 3.81 ± 0.01c 3.48 ± 0.04d 3.24 ± 0.01e
Corchorus olitorius 5.44 ± 0.65bc 5.76 ± 0.06b 6.67 ± 0.38a 4.80 ± 0.00cd 4.21 ± 0.01d 4.94 ± 0.03cd

Values are means of tree replicates, values with the same letter in a column are not significantly different from each other at p>0.05

Table 3: The mineral composition of vegetable samples.

The aflatoxin content of Vernonia amygdalina and Corchorus olitorius

The aflatoxin analysis conducted on two vegetables (Vernonia amygdalina and Corchorus olitorius) collected from different locations in Nigeria showed that they contain some levels of aflatoxin B1, B2, G1 and G2 which tend to increase with the storage time (Table 4). Aflatoxin B1 and B2 were recorded highest in at three month of storage while Aflatoxin G1 and Aflatoxin G2 were also detected highest in three and two month old samples. It was also observed that the location apart from the storage time also had significant effect on the aflatoxin contents of the sample (Table 4).

Duration Location Vegetables     Aflatoxin (µg/kg)
Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2
 Fresh Bodija Market Vernonia amygdalina 0.0000 ± 0.000g 0.0010 ± 0.000kl 0.0010 ± 0.000d 0.0000 ± 0.000m
Corchorus olitorius 0.0010 ± 0.000g 0.0010 ± 0.000kl 0.0005 ± 0.001d 0.0005 ± 0.001m
Oja-Oba Market Vernonia amygdalina 0.0000 ± 0.000g 0.0010 ± 0.000kl 0.0010 ± 0.000d 0.0010 ± 0.000lm
Corchorus olitorius 0.0010 ± 0.000g 0.0010 ± 0.000kl 0.0010 ± 0.000d 0.0010 ± 0.000lm
Moniya Market Vernonia amygdalina 0.0010 ± 0.000g 0.0020 ± 0.000jk 0.0020 ± 0.000cd 0.0010 ± 0.000lm
Corchorus olitorius 0.0010 ± 0.000g 0.0020 ± 0.000jk 0.0010 ± 0.000d 0.0020 ± 0.000kl
 One month Bodija Market Vernonia amygdalina 0.0100 ± 0.000f 0.0200 ± 0.000i 0.0010 ± 0.000d 0.0010 ± 0.000lm
Corchorus olitorius 0.0200 ± 0.000e 0.0300 ± 0.000d 0.0025 ± 0.001cd 0.0020 ± 0.000kl
Oja-Oba Market Vernonia amygdalina 0.0010 ± 0.000g 0.0030 ± 0.000j 0.0010 ± 0.000d 0.0025 ± 0.001k
Corchorus olitorius 0.0100 ± 0.000f 0.0010 ± 0.000kl 0.0020 ± 0.000cd 0.0035 ± 0.001j
Moniya Market Vernonia amygdalina 0.0010 ± 0.000g 0.0020 ± 0.000jk 0.0010 ± 0.000d 0.0030 ± 0.000jk
Corchorus olitorius 0.0010 ± 0.000g 0.0030 ± 0.000j 0.0010 ± 0.000d 0.0020 ± 0.000kl
 Two month Bodija Market Vernonia amygdalina 0.0200 ± 0.000e 0.0220 ± 0.000h 0.0040 ± 0.000bcd 0.0050 ± 0.000i
Corchorus olitorius 0.0285 ± 0.002b 0.0330 ± 0.000c 0.0060 ± 0.000bcd 0.0060 ± 0.000gh
Oja-Oba Market Vernonia amygdalina 0.0290 ± 0.001b 0.0345 ± 0.001b 0.0030 ± 0.000bcd 0.0050 ± 0.000i
Corchorus olitorius 0.0240 ± 0.001d 0.026 ± 0.0014f 0.0050 ± 0.000cd 0.0065 ± 0.001g
Moniya Market Vernonia amygdalina 0.0210 ± 0.000e 0.0230 ± 0.000gh 0.0025 ± 0.001cd 0.0035 ± 0.001j
Corchorus olitorius 0.0200 ± 0.000e 0.0240 ± 0.000g 0.0135 ± 0.015a 0.0055 ± 0.001hi
 Three month Bodija Market Vernonia amygdalina 0.029 ± 0.0014b 0.0305 ± 0.001d 0.0075 ± 0.001abcd 0.0110 ± 0.000d
Corchorus olitorius 0.036 ± 0.0014a 0.0405 ± 0.001a 0.0085 ± 0.001abc 0.0120 ± 0.000c
Oja-Oba Market Vernonia amygdalina 0.036 ± 0.0014a 0.041 ± 0.0014a 0.0055 ± 0.001abcd 0.0075 ± 0.001e
Corchorus olitorius 0.028 ± 0.000bc 0.0335 ± 0.002bc 0.0070 ± 0.000bcd 0.0095 ± 0.001f
Moniya Market Vernonia amygdalina 0.0265 ± 0.001c 0.0280 ± 0.000e 0.009 ± 0.0014abc 0.0135 ± 0.001b
Corchorus olitorius 0.0245 ± 0.001d 0.0265 ± 0.001f 0.0105 ± 0.001ab 0.0155 ± 0.001a

Values are means of tree replicates, values with the same letter in a column are not significantly different from each other at p>0.05

Table 4: The aflatoxin concentration of vegetable samples.

Discussion

This study reveals that storage time had significant effect on the nutrient and aflatoxin compositions of two selected vegetables Vernonia amygdalina and Corchorus olitorius, the most dominantly associated fungal stains with the dried samples of these vegetables are identified as Aspergillus flavus, Aspergillus niger, Aspergillus terreus, Aspergillus tamarii, Aspergillus parasiticus, Aspergillus fumigatus, Penicillium chrysogenum and Rhizopus nigricans. The presence of these organisms is not surprising as their source could be from soil, dust or the vegetable themselves at the point of harvest however, many of these fungi belongs to the group of aflatoxin producing fungi; this observation is in conformity to the reports of that reported many Aspergillus species as the common storage fungi affecting dried tomato and pumpkin leaf [27,28]. Aguoru et al. [29] also reported Aspergillus fungi as part of the major contaminants in the environment which causes spoilage of foods and many agricultural produce [29].

It was observed in this study that the aflatoxin contaminations in samples increased with concurrent fungal incidence in the dried vegetables especially at the third month of storage as earlier observed by who reported progressive increase in species of fungi in stored sundried plantain chips and coco yam chips respectively [30,31]. This also follow the reduction in the proximate composition of the samples which could be due to the deteriorating activities by the fungi as substrate for metabolism similar to the observation, when they worked on some indigenous green leafy vegetables [32-34]. The quantity of crude protein, fat, crude fibre and ash in both samples decreased with an increasing storage period except carbohydrate which increased with the storage period. This could be as a result of reduction in moisture content during storage as suggested, who also reported that carbohydrate in Moringa leaves increased on air drying compared to the fresh form [32]. Generally, both vegetables were rich in Potassium, Calcium, Phosphorus and Sodium over Iron, Zinc and Magnesium, but their nutrient composition varied cross location, this is could be as a result of different factors such as geographic locations, or soil fertility or its fecundity [35,36].

The fresh samples were observed to contain lower aflatoxin contaminations as compared to the stored samples; this is in agreement with the report of who reported less aflatoxins contents in fresh pumpkin leaves and Spinaches between the range 0.07-7.32 μg/kg as compared to the stored samples [37-39]. However, aflatoxins B1 and B2 were detected higher than the aflatoxins G1 and G2 in the samples, which are generally higher in stored samples that fresh ones, this could be due to the fact that the phytochemicals such as flavonoids, saponins and alkaloids present on the fresh plants are more potent as antifungal biocide than in dried samples [40,41]. 27 found 60% of dried red chili pepper contaminated with up to 19.45 μg/kg aflatoxins but detected no aflatoxin in fresh Baobab and Okra leaves.

Several abiotic factors influence the growth of moulds on dried vegetables; they are relative humidity and temperature together with the moisture content of the product and storage conditions [42-44]. The main factor responsible mycotoxin production is the interaction between the fungi, its host, and the environmental condition as the concentrations of the aflatoxin vary with the location [45]. The level of fungi infestations can be compounded by insect damage, stress condition at pre-harvest, harvest and post-harvest stages of vegetable production [44]. Other possible source of fungi infestation can result from inappropriate handling and storage methods often associated with poor hygiene and also the methods of processing, packaging and storage can also have great effect on fungal contamination. The climatic and environmental conditions are highly favorable for the propagation of fungi especially the genera Aspergillus spp., Penicillium spp., Mucor spp. and Rhizopus spp. that produce and release a lot of spores [46]. From this study, it was observed that dried vegetable can also serve as substrates for aflatoxin producing fungi and these products are exposed to environmental condition that favors the fungal growth and development. It has been reported that ingestion of foods that are contaminated with high concentration of mycotoxins can lead to much greater health risks [47].

Conclusion

It was observed from this study that storage time can affect the nutritional value of dried bitter leaf and jute vegetables and as well may increase their aflatoxin contaminations due to associated fungi. However from this study it was observed that the aflatoxin concentrations in the studied vegetables were generally within the permissive level of 20 μg/ kg for adults as stipulated in most countries. The presence of aflatoxin in these vegetables necessitates the involvement of good agricultural practices, hygienic processing and handling with good storage practices and facilities. There is also a need for central risk assessment evaluation on all commodities to be consumed to prevent fungal growth in stored vended food and this could also be achieved through the application of strict hygienic measures during processing and storage periods.

References

Citation: Segun GJ, Olanrewaju GO, Michael DA, Omolola OO (2018) Effect of Storage Time on Nutrient, Biodeteriorating Fungi and Aflatoxin Contents of Bitter Leaf (Vernonia amygadalina) and JUTE (Corchorus olitorius). J Microb Biochem Technol 10: 49-55.

Copyright: © 2018 Segun GJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.