Motivation represents one of the most important variables in sport. A large number of studies incorporating the SDT framework in the sport domain have confirmed that SDT is appropriate to understanding and promoting optimal motivation in sport. The purpose of this study was to determine the validity and reliability of sport motivation questionnaire. For selecting our sample we use available random sampling, so 200 participants were randomly selected for both male and female of professional athletes on the field of volleyball team, basketball, football, handball, futsal and then completed the questionnaires in a voluntary manner. Cronbach's alpha was used to calculate the reliability and confirmatory factor analysis test was used for studying the validity of the questionnaire. Findings showed that a Cronbach's alpha level for sport motivation was 0.80, which indicates the reliability of the variable is relatively good. And it should be mentioned that Sport motivation scale models used in society of professional athletes of Iranian team was relevant.