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Abstract

The Immunogenicity of Peptoid-Protein Conjugates

Allison Case, Angela Desmond, Daniel Lopes, Kelly Dye, Kelly Mapes, Stephen Ruback, Iliodora Pop, Jiyeun Kate Kim, Pavitra Chakravarty, Joan E. Smallshaw, Laurentiu M. Pop and Ellen S.Vitetta

We demonstrate that a peptoid composed of five monomers and attached via a maleimide linker to a carrier protein elicits anti-peptoid, anti-linker and anti-carrier antibodies in rabbits. Specific anti-peptoid antibodies were affinity purified and used to reproducibly retrieve three specific peptoid-coupled beads from 20,000 irrelevant peptoid-beads using magnetic screening.