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Spectral Analysis and Antibacterial Activity of Methanol Extract of Roots of Echinops echinatus and its Fractions

Muhammad Younus, Farrakh Zia Khan, Sabira Sultana and Hafiz Muhammad Asif

Objective: The root of Echinops echiantus L. (Asteraceae) is reported to have great medicinal value. The aim of the present study was to determine the chemical profile of methanol extract of Echinops echiantus by two different spectral analyses which will be useful for its proper identification when therapeutically used. Isolated compounds and crude methanol extract were screened for antibacterial activity.

Methods: Spectral analysis was done by different analytical methods such as Infrared spectroscopy (FTIR) and Ultraviolet spectroscopy. The in vitro antibacterial activity of E. echinatus root extracts was studied by the hole plate diffusion method against several human pathogenic gram-positive and gram-negative bacteria.
Results: Five compounds were isolated from crude methanol extract using column chromatography and characterized by techniques like Infrared spectroscopy (FTIR) and Ultraviolet spectroscopy. Compounds showed different Rf values ranging from 67 to 9.4. All the five isolated compound and crude methanol extract showed significant antibacterial activity against all strains. However, the maximum activity was shown by compound Ee-4, Ee-1, Ee-3 against Staphylococcus aureus (17.0 mm), (15.3 mm), (12.3 mm) respectively. While Ee-5 give maximum activity against E. coli (13.5 mm).

Conclusion: The result of this study suggested that methanol extract of Echinops echiantus root and its isolated compounds exhibited marked antibacterial activity. Therefore, the results of this study may act as biochemical markers for this medicinally important plant in the pharma industry and plant systematic studies.