Sponges are host organisms for various symbiotic microorganisms such as Archea, Bacteria, Cyanobacteria and Microalgae. Sponges associated microorganisms are sources of wide variety of useful natural products like antibiotics, anti-inflammatory, antioxidant, antiviral, antifouling and cytotoxic compounds. Nearly 60 isolates of action bacteria were found to be associated with 6 species of marine sponges collected at various locations. The isolates were screened for antimicrobial activity against 6 pathogenic bacteria and 4 pathogenic fungi. Among 60 isolates, 15 isolates showed antibacterial activity and 6 isolates showed antifungal activity. Among active isolates, isolate no.42 showed highest antimicrobial activity against all the pathogenic bacteria and fungi studied and it was identified as streptomyces species.