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Incidence of Listeria monocytogenes in Meat Product Samples by Real-Time PCR

Miroslava Kacániová, Maciej Kluz, Jana Petrová, Martin Mellen, Simona Kunová, Peter Hašcík and Lubomír Lopašovský

The aim of this study was to trace a contamination of meat products with Listeria monocytogenes. Step One real time Polymerase Chain Reaction (PCR) was used. We used the PrepSEQ Rapid Spin Sample Preparation Kit for isolation of DNA and MicroSEQ® Listeria monocytogenes Detection Kit for the real-time PCR performance. We found out the strains of Listeria monocytogenes in hundred samples of meat products with no incubation. There was Internal Positive Control (IPC) in 40 samples. Our results showed that the real time PCR assay tested in this study might detect Listeria monocytogenes in meat product samples with no incubation sensitively.