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Identification of Xylanase Signal Peptide in Culture Supernatant of Clostridium saccharoperbutylacetonicum Strain N1-4 Cultured on Delignified Rice Straw

Eri Kubota, Keiji Kiyoshi, Kosuke Nobuki, Toshimori Kadokura, Atsumi Nakazato and Shunichi Nakayama

The butanol-producing Clostridium saccharoperbutylacetonicum strain N1-4 abundantly secreted xylanase into the culture supernatant when cultured on delignified rice straw. The xylanase signal peptide was identified based on its N-terminal amino acid sequence and was presumed to be secreted by the Sec-system. The presence of xylanase in the medium indicated that the strain was capable of utilizing xylan as a substrate. Also, butanol production obtained using a medium supplemented with xylan was comparable to that obtained using the same strain cultured on a medium supplemented with glucose. The signal peptide facilitated the secretion of exogenous cellulases expressed by butanol-producing strains and promoted butanol production from dignified rice straw.