Blood transfusion is an indispensable component of health care. It contributes to saving millions of lives each year in both routine and emergency situations, permits increasingly complex medical and surgical interventions and dramatically improves the life expectancy and quality of life of patients with a variety of acute and chronic conditions. to analyze and evaluate the reasons for donor deferral at ESIC hospital blood bank which has been recently opened up, so that temporarily deferred donors with corrective reasons can be identified, properly informed and guided to improve their quality of blood for future donation. Out of all the donors 1600 (90.3%) were eligible for donation and 171 (9.7%) blood donors were deferred. Temporary deferrals (77.8%) were more common than permanent deferral (22.2%). The most reasons for deferral in blood donors were Anaemia (40.9%), Hypertension (52.6%), Antibiotic therapy (10.5%), Previous donation (5.3%) and Malaria (4.5%). Thus, it is important to evaluate the reasons of whole blood donor deferral to guide the recruitment and retention efforts in newly started blood bank.