Journal of Plant Pathology & Microbiology

Abstract

Characterization and Confirmation of Corchorus Golden Mosaic Virus Associated with Jute in Bangladesh

Hasan MM, Meah MB, Ali MA, Okazaki K and Sano Y

Yellow mosaic disease is a major limiting factor for jute (Corchorus capsularis L.) cultivation in Bangladesh. We have cloned and sequenced three isolates of Corchorus golden mosaic virus (CoGMV) collected from different regions in Bangladesh. DNA A sequence of CoGMV-[BD:Mym:10] (BD1] shared highest identity (94.2%) with the Vietnam isolate of CoGMV, whereas DNA B shared a lower level of sequence identity (<73%) with the CoGMV isolates reported from Vietnam and India. Complete genome sequences of CoGMV-[BD: Ran: 10] [BD2] and CoGMV-[BD:Din:10] [BD3] showed at least 97% sequence identity with Indian isolates of CoGMV. The fact that the examined DNA A components of three isolates of CoGMV lack the AV2 open reading frame, indicated that BD1-3 share genetic features of New World begomoviruses. The pathogenicity of CoGMV-[BD:Din:10] [BD3] isolate was confirmed by agroinoculation and infectious clones of DNA A and DNA B induced characteristic yellow mosaic symptoms in jute plants. This is a first experimental demonstration of Koch’s postulate for a begomovirus associated with jute yellow mosaic disease