Journal of Biomolecular Research & Therapeutics

Abstract

Biological Evidences of Hepatocellular Carcinoma Treatment with 1,3,4-oxadiazole-2-thiol as Anticancer Agent

Mohamed S Gabry, Galal H Elgemeie, Nahed S Basseli, Samuel T Melek, Saadia E Hafez, Omar A Farid and Shimaa S Abdelhady

Purpose: In recent years, identification of novel potent, selective, and less toxic anticancer agents remains one of the most pressing health problems. This research aimed to in vivo illustration of 1,3,4-oxadiazole-2-thiol (OXD-T) anticancer activities as a discovery of the treatment of Hepatocellular Carcinoma (HCC) of Albino rats.

Methods: Hepatocellular carcinoma was induced by 3,3’-Diamino benzidine for three months, three times per week. The post treatment of HCC induced rats was carried out with OXD-T therapeutic (300 mg/kg. b.wt.) and half therapeutic doses (150 mg/kg b.wt.) of administration. Biochemical parameters and comet assay were assessed to evaluate the efficiency of OXD-T treatment on the HCC induced animal.

Results: The administration of 1,3,4-oxadiazole-2-thiol (OXD-T) with therapeutic dose and half therapeutic dose to hepatocellular carcinoma (HCC) induced rats affected the biochemical values as biomarkers; prothrombin induced by vitamin K absence-II (PIVKA-II) and lactate dehydrogenase LDH. Also, serum enzymes; AST, ALT, GGT and Albumin. Furthermore, OXD-T affected the DNA fragmentation parameters (tail length, tail moment, % DNA in tail and % DNA in head of comet). OXD-T therapeutic administration revealed highly significant decreases in the biochemical values and DNA fragmentation parameters more than half dose of OXD-T treatment.

Conclusion: OXD-T antimetabolite with different therapeutic doses of administration affected the growth of hepatocellular carcinoma.