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Abstract
Analysis of Microbial Biochemical Inducting Nutritional Contents in Barley
Anam Yousaf, Yaseen Ashraf, Nasim Ahmad Yasin, Asma Ibrahim, Aqeel Ahmad, Waheed Ullah Khan, Zoobia Bashir, Waheed Akram and Zarish Noreen
Current study focuses the biochemical analysis of the metabolites of Acetobacter aceti to explore bioactive compounds inducing nutritional elements (including vitamins) in barley seeds. Thirteen chemical compounds were isolated using bioactivity guided assay and chromatographic analyses of bacterial extracts. The, principal component analysis (PCA) indicated the most active biochemicals among them. It was resulted into five chemical compounds (i.e. Mevalonic Acid, Quinolinic Acid, Pyridoxic Acid, p.Aminobenzoate and and α-Oxobutanoic acid), which were highly associated with increased quantities of phytosterols, phenols and tested vitamins. A solvent system consisting upon chloroform:ethanol (4:1) was recommended as the best solvent system for extraction of active compounds from bacterial metabolites. Moreover, the mechanisms have been proposed according to which plant pathways were directed towards elevated biosynthesis of nutritional elements. Significant quantities of Mevalonic Acid were concluded into induction of is