Journal of Microbial & Biochemical Technology

Abstract

A Non Invasive Technique to Assess Mucosal Immunity in Healthy Population by Measuring Immunoglobulin Receptor Expression on Viable Colonocytes

Bamola VD, Sharma N, Abhipray Gahlowt, Panigrahi P and Chaudhry R

Human gut plays a vital role in the regulation of immune function, mucosal defense and homeostasis. Gut epithelial cells function as an immune cell and express receptors for microbial-associated molecular patterns. The gut epithelium undergoes constant and rapid renewal and some of these cells are exfoliated into the fecal stream. These cells are an important source of macromolecules, which provides a patho-physiological profile of the colonic epithelium. Most of the methods to harvest colonic epithelial cells are highly invasive and involve endoscopy and biopsy. Researchers suggest that studies of gastrointestinal pathophysiology are not feasible by biopsies in neonates and paediatric population. Therefore, isolation of theses exfoliated viable colonocytes from human stool is a noninvasive as well as a highly convenient approach that can be used for diagnostic and research purposes. A very few studies are available across the globe and no study is available from India of using this non-invasive techniques to recovered viable colonocytes in healthy population. For the first time we are reporting the results of the study on healthy Indian population where we recovered viable colonocytes from the stool samples using this non-invasive approach (Cell Sampling Recovery Method) and assessed immunoglobulins (IgA & IgG) receptors expression by Flowcytometry using specific florochrom conjugated antibodies. No study is available which provides the normal reference range of IgA and IgG receptor concentration on viable colonocytes in healthy Indian population. In this study we recruited 25 healthy children and 25 healthy adults from North India and provided the range of IgA and IgG receptor concentration on viable colonocytes for both groups. Results indicated that the difference in the mean IgA and IgG receptor concentration was statistically significant in both groups.